Phospholipids in ram semen: metabolism of plasmalogen and fatty acids

Phospholipids in ram semen: metabolism of plasmalogen and fatty acids.

Plasmalogen in ram semen, and its role in sperm metabolism.

Whole semen can be stored successfully under both aerobic and anaerobic conditions, owing to the presence of glycolysable carbohydrate in the seminal plasma. However, if mammalian spermatozoa are separated from the seminal plasma by centrifuging and washing, they can maintain their motility only in the presence of oxygen. From this it has been inferred that when spermatozoa are deprived of fruc...

Amino acids in ram testicular fluid and semen and their metabolism by spermatozoa.

1. The testis of the ram secretes considerable amounts of amino acids (200mumoles/day) into the fluid collected from the efferent ducts. The principal amino acid in this testicular fluid is glutamate, which is present in concentrations about eight times those in testicular lymph or in blood from the internal spermatic vein. 2. The concentration of glutamate in seminal plasma from the tail of th...

Incorporation of Photosensitive Fatty Acids into Phospholipids

In an approach to the study of phospholipid-protein interactions in biological membranes, the photoactivable fatty acids, w-(m-azidophenoxy)-undecanoic acid (I) and o-(m-diazirinophenoxy)-hexadecanoic acid (11), were incorporated biosynthetically into the phospholipids of the Escherichia coli fatty acid auxotroph, strain K1060-B5. The extent of incorportation of the two fatty acids was 43% and ...

Storage of ram semen.

Storage of ram semen in liquid and frozen state, the diluents used for both methods, processing, cooling, freezing and thawing of semen are reviewed. Factors influencing the fertility of stored semen and methods used for improvement are discussed, and fertility results of long-term frozen stored ram semen are also given.